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Encompassing Amino Acids: 2-790(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction was performedin 50 mM Tris pH 7.4, 1 mM DTT, 0.5 mMEDTA, 500 nM Ub-AMC, and variousamount of A20 in a volume of 50 µL.Reaction was incubated at 37°C for 30 minand fluorescent signal was measured atexcitation of 360 nm, and emission at 460nm.
Description: Human A20, also known astumor necrosis factor- induced protein 3(TNFAIP3) or OTU domain-containingprotein 7C (OTUD7C), GenBank AccessionNo. NM_006290, amino-acids 2-790(end)with an N-terminal FLAG-tag and C-terminalHis-tag, MW=91 kDa, expressed in Sf9insect cells via a baculovirus expressionsystem.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, and 20% glycerol
Genbank: NM_006290
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag, C-terminal His-tag
Uniprot: Q9UHC1
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Shi CS, Kehrl JH. (2010). Autophagy 6(7):986-7.
2. Tavares, R.M., et al. (2010). Immunity 33(2):181-9.
3. Hymowitz, S.G., Wertz, I.E. (2010). Nat. Rev. Cancer 10(5):332-41
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