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Applications: As an exopeptidase, ACE2 is useful for the study of enzyme kinetics and to screen for inhibitors. As a cell receptor for coronaviruses infection, including 2019-nCoV, ACE2 is useful for the study of protein-protein interactions.
Assay Conditions: ACE2 exopeptidase activity was measured in a 50 µl reaction containing L-Phe quantification reagents in 50 mM Tris pH 8.0, as previously described. Fluorescence intensity was kinetically recorded at 555 nm excitation/585 nm emission, and net initial rates, measured in RFU/min, were used to plot the results.
Description: Human Angiotensin converting enzyme 2 (ACE2) also known ACEH, GenBank Accession No. NP_068576.1, a.a. 18-740, with a C-terminal His-tag, expressed in a HEK293 expression system. MW=85 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris pH 8.0, 110 mM NaCL, 2.2mM KCl, 200 mM Imidazole and 20% glycerol.
Genbank: NP_068576.1
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: C-terminal His-tag
Uniprot: Q98BYF1
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Oudit, G.Y., et al., Trends in Cardio. Med. 2003 Apr;13(3):93-101.
2. Donoghue, M., et al., Circulation Research. 2002 Sep;87:e1-e9
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