Target: GRP78 (Bip) .
Nature: Recombinant.
Swiss-Prot: P11021.
Biological Activity: ATPase active.
Expression System: E. coli.
Amino Acid Sequence: MKLSLVAAMLLLLSAARAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL.
Purification: Affinity Purified.
Purity: >90%.
Storage Buffer: 20mM Tris/HCl pH7.5, 50mM KCl, 5mM MgCl2, 1mM DTT.
Protein Size: ~78 kDa.
Conjugate: His tag.
Cellular Localization: Endoplasmic Reticulum Lumen, Melanosome.
Scientific Background: GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia (Barnes), to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).
References: 1. Cho S., et al (2007) Mol Cell Biol. 27(1): 368-83.2. Yang Y., et al. (1998) J Biol Chem. 273: 25552-25555.3. Luo S., et al (2006) 26(15): 5688-97.4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314.5. Koomagi R., et al. (1999) Anticancer Res. 19: 4333-4336.6. Laquerre S., et al. (1998) J. Virology. 72: 4940-4949.7. Dong D., et al. (2005) Cancer Res. 65(13): 5785-91.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.
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