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Encompassing Amino Acids: 2-503 (end)
Applications: Useful for binding studies to investigate protein-protein interactions in the EZH2 complex. Also suitable as an immunogen or as a standard for Western blotting.
Description: Human Adipocyte Enhancer binding protein 2 (AEBP2), GenBank Accession No. NM_153207, a.a. 2-503 (end) with N-terminal His-tag, MW=53.6 kDa, expressed in Sf9 cells via a baculovirus expression system. Runs at ~70 kDa on SDS-PAGE gels. *Note: Protein runs aberrantly in SDS-PAGE gels, and has been confirmed by Western blotting.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl pH 8.0, 110mM NaCl, 2.2 mM KCl and 20% glycerol
Genbank: NM_153207
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q6ZN18
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Kim, H., et al., PLoS One. 2011; 6(9):e25174.
2. Cao., R., et al., Mol. Cell. 2004 Jul 2; 15(1): 57-67.