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Application Note: Anti-AHSP Antibody has been tested in western blot and immunohistochemistry and is suitable for immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures and ELISA. Specific conditions for reactivity should be optimized by the end user.
Concentration Value: 115 mg/mL
ELISA Dilution: 1:2,000 - 1:10,000
Immunohistochemistry Dilution: 1:8000
Western Blot Dilution: 1:500-1:1000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Anti-AHSP Antibody was produced from whole rabbit serum prepared by repeated immunizations with the full length human AHSP protein.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Anti-AHSP Antibody is directed against the human AHSP protein. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest cross reactivity with human and mouse. Cross-reactivity with AHSP from other sources have not been determined.
Background: AHSP Antibody detects Alpha hemoglobin stabilizing protein (AHSP). AHSP acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. AHSP binds free a-globin to promote its folding and inhibit its ability to produce damaging reactive oxygen species. Reduced AHSP levels correlate with increased severity of b-thalassemia in some human cohorts.
Low Endotoxin: No
Other: User Optimized
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