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Applications: Useful as a substrate in kinase assays.
Description: Human Akt1 (GenBank Accession No. NM_001014432), full length (a.a. 1-480) with N-terminal His-tag, MW=60.2 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.2mM KCl, 10% glycerol, and 3 mM DTT.
Genbank: NM_001014432
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P31749
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: 1. Alessi et al., Current Biology 8: 69, 1998. 2. Cohen et al., FEBS Lett. 410: 3, 1997.Application Reference:Koeberle, A., et al. Proc Natl Acad Sci USA. 2013 Feb 12;110(7):2546-51. "Arachidonoyl-phosphatidylcholine oscillates during the cell cycle and counteracts proliferation by suppressing Akt membrane binding"
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