Application Note: Anti-Alpha-2-Macroglobulin has been tested by western blot and is suitable to be assayed against 1.0 ug of a2-Macroglobulin [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:32,000 to 1:160,000 of the reconstitution concentration is suggested for this product.
Concentration Value: 82 mg/mL
ELISA Dilution: 1:20,000 - 1:100,000
Immunohistochemistry Dilution: 1:500 - 1:2,500
Western Blot Dilution: 1:2,000 - 1:10,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: a2-Macroglobulin [Human Plasma]
Physical State: Lyophilized
Purity and Specificity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified a2-Macroglobulin [Human Plasma]. Cross reactivity against a2-Macroglobulin from other sources is unknown.
Background: Alpha-2-Macroglobulin is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. Alpha-2-Macroglobulin has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Low Endotoxin: No
Other: User Optimized
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