Alpha Synuclein Monomers: Biotinylated (C-terminus)

Human Recombinant Alpha Synuclein Monomers: Biotinylated (C-terminus)

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Target: Alpha Synuclein Biotinylated (C-terminus)

Nature: Recombinant

Swiss-Prot: P37840

Expression System: E. coli

Protein Length: 155 aa

Amino Acid Sequence: MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVH GVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQL GKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEAGLNDIFEAQK IEWHE

Purification: Ion-exchange Purified

Purity: > 95% based on SDS-PAGE and nanodrop analysis.

Storage Buffer: PBS pH 7.4

Protein Size: 16.27 kDa

Conjugate: C-terminal 15 amino acid tag: biotinylated

Scientific Background: A 15 amino acid tag on the C-terminal tail of Alpha Synuclein facilitates site-specific covalent biotinylation (1). Biotinylation of purified alpha synuclein allows for many biological applications, such as monitoring and detection using streptavidin-based conjugates (1, 2, 3). Our biotinylated monomers are also used to generate pre-formed fibrils in-vitro.

References: 1.Fairhead & Howarth. 2015. Site-specific biotinylation of purified proteins using BirA. Methods in Molecular Biology. DOI: 1007/978-1-4939-2272-7_122.Hallacli et al. 2022. The Parkinson’s disease protein alpha synuclein is a modulator of Processing-bodies and mRNA stability. Cell. DOI: 10.1016/j.cell.2022.05.0083.Li et al. 2019. Naturally occurring antibodies isolated from PD patients inhibit synuclein seeding in vitro and recognize Lewy pathology. Acta neuropathologica. DOI: 10.1007/s00401-019-01974-5.

Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• SKU: STRSPR-507XG
• Manufacturer: Stressmarq Biosciences
• Manufacturer SKU: SPR-507XG
• Package Unit: 500 µg x2 (@5mg/ml)
• Quantity Unit: PAK
• Reactivity: Human
• Application: Western Blotting, In Vivo Assay, SDS-PAGE