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Applications: WB (1:1000)
Assay Conditions: Western blot analysis using the antibody directed against ASH2
Figure 1A: Western blot was performed using the antibody against hASH2 (cat. #25209) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk on nuclear extracts from HeLa cells (upper panel: lane 1) and ectopically expressed FLAG-tagged ASH2 (ASH2-F, upper panel: lane 2). The lower panel shows the same Western blot with an anti-FLAG antibody. The molecular weight marker is shown on the left.
Figure 1B: Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 µg) with the antibody against ASH2 (cat. #25209) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
Background: ASH2 (UniProtKB/Swiss-Prot entry Q9UBL3) is a component of the SET1/ASH2 histone methyltransferase (HMT) complex. This complex specifically methylates K4 of histone H3, thereby activating transcription. Methylation of K4 is blocked by premethylation of the neighboring K9, a repressor of transcription. This indicates that the SET1/ASH2 HMT complex mediates the crosstalk between K9 methylation and K4 methylation. ASH2 plays a role in hematopoiesis and may be associated with some kinds of leukemia.
Concentration: 100 µl
Description: Polyclonal antibody raised in rabbit against human Ash2 (absent, small, or homeotic 2), using a recombinant protein
Format: Aqueous solution
Formulation: Whole antiserum from rabbit containing 0.05% azide
Immunogen: recombinant protein
Purification: Whole serum
Storage Stability: Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month.
Uniprot: Q9UBL3
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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