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Applications: Useful for studying expression of CD19 on the cell surface by flow cytometry.
Assay Conditions: One million CD19 CHO cells (BPS Bioscience #79561) were blocked and stained with 1 µg of anti-CD19 antibody, FITC-labeled (BPS Bioscience #101863) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. CD19 CHO cells (green) were compared to parental CHO-K1 cells (blue). Y-axis represents the cell count, while X-axis shows FITC intensity.
Background: CD19 (also known as Cluster of Differentiation 19, B-lymphocyte surface antigen B4, or CVID3) is a glycoprotein expressed at the surface of B lymphocytes through most phases of B cell maturation. It is strictly required for B cell terminal differentiation. Most B cell malignancies express normal to high levels of CD19, making it a nearly ideal target for cancer immunotherapy. CD19 was the target of the first approved CAR-T cell therapy. Studies of CD19 function and expression patterns will continue to broaden our knowledge and support possible applications in cancer therapy.
Cross Reactivity: This antibody recognizes human CD19. It has not been tested in other species.
Description: This anti-CD19 antibody is a purified recombinant antibody, which is labeled with fluorescein isothiocyanate (FITC). This antibody has been tested by flow cytometry for specific binding to human CD19 on the surface of CD19 CHO Recombinant Cell Line (BPS Bioscience #79561).
Format: Aqueous buffer solution.
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.
Host Cell Line: HEK293
Purification: Protein A affinity chromatography from HEK293 supernatant.
Purity: ≥90%
Storage Stability: At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.
Uniprot: P15391
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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