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Applications: The anti-CD19 IgG format antibody can be used for labeling cells expressing CD19 for flow cytometry and immunofluorescence microcopy.
Assay Conditions: Purified human his-tagged CD19 was bound to clear 96-well nickel plate overnight in 4°C (1 µg/mL). Upon washing and blocking wells for 1 hour at room temp, serial dilutions of anti-CD19 (200 nM to 0nM in 3-fold dilutions) were incubated in each well for 1 hr at room temp (slow shaking). Next, wells were washed and incubated with anti-human IgG-HRP for 1 hour at room temp (slow shaking) then washed again. For detection, the wells were incubated with Colorimetric HRP Substrate (BPS Bioscience #79651) for 1-10 minutes until a blue color developed in the positive control. The reaction was then quenched with equal volume of 1N HCL and absorbance read at 450 nm.
Description: This purified recombinant monoclonal antibody is a human Anti-CD19 IgG which recognizes CD19 antigen. The variable regions for this antibody are based on an FDA-approved drug. This antibody has been tested for specific binding affinity to purified human CD19 protein (BPS Bioscience, #101015) in a colorimetric ELISA binding assay.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol
Purification: Protein A affinity purification from HEK293 cells.
Purity: ≥90%
Storage Stability: At least 12 months at -80°C.
Supplied As: Aqueous buffer solution.
Uniprot: P15391
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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