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Applications: Useful for studying expression of CD235a on the cell surface in flow cytometry assay. For flow cytometric staining, the suggested use of this reagent is 5 µl (1 µg antibody) per million cells in 100 µl staining volume.
Assay Conditions: One million CD235a CHO recombinant cells (BPS Bioscience #78353>) were blocked and stained with 1 µg of anti-CD235a antibody, PE-labeled (BPS Bioscience #101675) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. CD235a CHO recombinant cells (green) were compared to parental CHO-K1 cells (blue). Y-axis is the cell count. X-axis is PE intensity.
Concentration: The concentration will vary with each lot; the exact value will be provided with each shipment.
Cross Reactivity: This antibody recognizes human CD235a. It has not been tested with other species.
Description: This anti-CD235a antibody is a purified recombinant antibody, which is labeled with R-Phycoerythrin (PE). This antibody has been tested in flow cytometry assay for specific binding to human CD235a on the surface of CD235a CHO Recombinant Cell Line (BPS Bioscience #78353).
Format: Aqueous buffer solution
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain protein stabilizer.
Host Cell Line: HEK293
Purification: Protein A affinity chromatography from HEK293 supernatants.
Purity: ≥90%
Storage Stability: At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.
Uniprot: P02724
Warnings: Do not freeze. Protect from light.
Biosafety Level: Not applicable (BSL-1)
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