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Applications: This antibody is useful for activation of CD28 costimulatory pathway in T cells
Assay Conditions: Serial dilutions of anti-CD3 (BPS Bioscience, #71274) and CD28 antibodies were added to the IL-2 reporter-Jurkat cells, then incubated at 37°C incubator for 16 hrs. After the treatment, perform Luciferase assay using One-Step Luciferase assay system (BPS Bioscience, #60690)
Background: The T cell-specific receptor CD28 is an important regulator of the immune system. The ligation of CD28 and its ligands CD80/CD86 on the APC provides a critical costimulatory signal alongside T cell receptor (TCR) for T cell activation. The costimulatory signal can also be delivered by the interaction between CD28 and its agonist antibody.
Description: Recombinant monoclonal antibody recognizing human CD28. This antibody binds to CD28 on T cells and promotes T cell response. Not tested in other species. This antibody crosslinked with FcGR2B is useful for activation of CD27 costimulatory pathway in T cells. This antibody has been humanized to reduce immunogenicity and improve the therapeutic half-life.
Format: Aqueous buffer solution.
Formulation: 8 mM Na Phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol
Purification: Protein A affinity chromatography from HEK293 supernatants.
Storage Stability: Stable for at least 12 months at -80°C.
Uniprot: P10747
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Takemura, S., et al., FEBS Letters, 2000; 476: 266-271.