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Application: Useful for studying cell surface expression of DLL3 by flow cytometry.
Assay Conditions: One million DLL3 CHO cells (#78882) were blocked and stained with 1 µg of Anti-DLL3 Antibody, PE-Labeled (#102503) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. DLL3 CHO cells (green) were compared to parental CHO-K1 cells (blue). Y-axis indicates the cell count. X-axis represents PE intensity.
Background: DLL3, also known as delta like ligand three, is a Notch ligand characterized by a DSL domain, transmembrane region, and a series of EGF (epidermal growth factor) repeats. Notch ligands can participate in trans-interactions (interaction with Notch receptor on a different cell) and cis interactions (interaction with Notch receptor within the same cell) to activate or inhibit Notch signaling, respectively. DLL3 exclusively functions to inhibit Notch signaling through cis inhibition. While DLL3 expression is limited in healthy tissue, high expression levels of DLL3 are found in various cancers including small cell lung cancer (SCLC), where it plays an oncogenic role. Relieving DLL3-mediated inhibition of Notch signaling may serve as a therapeutic avenue, with drugs being developed to target DLL3 as a possible lung cancer therapy (example: rovalpituzumab tesirine).
Cross Reactivity: This antibody recognizes human DLL3. It has not been tested in other species.
Description: This anti-DLL3 (delta like ligand three) antibody is a purified monoclonal recombinant antibody, which is labeled with R-Phycoerythrin (PE). This antibody has been tested by flow cytometry for binding to human DLL3 on DLL3 CHO Cell Line (#78882).
Format: Aqueous buffer solution.
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.
Purification: Protein A affinity chromatography from HEK293 supernatants.
Purity: ≥90%
Storage Stability: At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.
Warnings: Avoid freeze/thaw cycles. Protect from prolonged exposure to light.
Biosafety Level: Not applicable (BSL-1)
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