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Applications: ELISA (1:500 - 1:1000)
WB (1:1000)
Assay Conditions: Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse G9a (Cat. No. 25224). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:30,000.Western blot analysis using the antibody directed against G9a
Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) cells with the antibody against mouse G9a (Cat. No. 25224), diluted 1:1,000 in BSA/PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (138 kDa) is indicated on the right.
Background: G9a (UniProtKB/Swiss-Prot entry Q96KQ7) is a member of the Set 1 family of histone methyltransferases. It preferentially methylates K9 and K27 of histone H3. Methylation of H3K9 represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. G9a is a part of the E2F6.com-1complex.
Concentration: 100 µl
Description: Polyclonal antibody raised in rabbit against mouse G9a (Protein G9a), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.
Format: Aqueous solution
Formulation: Whole antiserum from rabbit containing 0.05% azide
Immunogen: synthetic peptide
Purification: Whole serum
Storage Stability: Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month.
Uniprot: Q96KQ7
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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