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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: An ELISA binding assay was performed with this antibody and its target protein, GFRAL (BPS Bioscience #101013). The antibody was coated onto an assay plate overnight at 2 µg/ml at 4°. On the next day, the plate was washed on blocked. Then, serial dilutions of the target protein were added (0 to 200 nM) for 1 hour at room temperature. After washing, HRP-Streptavidin was incubated for 30 minutes at room temperature. Following another wash step, colorimetric HRP substrate was added and quenched with 1N HCL. Then we measured absorbance on a plate reader at 450 nm. EC50 = 2.4 nM
Description: Recombinant anti-GFRAL (GDNF Family Receptor alpha Like) monoclonal antibody which recognizes GFRAL. This recombinant antibody has been tested for specific binding to purified recombinant human GFRAL (BPS Bioscience #101013) in an ELISA binding assay.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Purity: ≥90%
Storage Stability: At least 12 months at -80°C.
Uniprot: Q6UXV0
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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