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Applications: ELISA (1:1000)
Assay Conditions: Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human HMG2L1 (Cat. # 25290). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:34,500.
Background: HMG2L1 (UniProtKB/Swiss-Prot entry Q9UGU5) is a negative regulator of Wnt/beta-catenin signaling during development
Concentration: 100 µl
Description: Polyclonal antibody raised in rabbit against human HMG2L1 (High mobility group protein 2-like 1) using two KLHconjugated synthetic peptides containing a sequence from the N-terminal and central region of the protein, respectively.
Format: Aqueous solution
Formulation: Whole antiserum from rabbit containing 0.05% azide
Immunogen: synthetic peptide
Purification: Whole serum
Storage Stability: Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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