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Applications: ELISA (1:200)
Assay Conditions: Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human KDM4D (cat. No. 25300). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:12,750.
Background: KDM4D (UniProtKB/Swiss-Prot entry Q9H3R0), also known as JMJD2D, is a histone demethylase that specifically demethylates Lys-9 of histone H3, thereby playing a central role in histone code. KDM4C does not demethylate histone H3K4, H3K27, H3K36 nor H4K20. It demethylates both di- and trimethylated H3K9, while it has no activity on monomethylated residues.
Concentration: 100 µl
Description: Polyclonal antibody raised in rabbit against human JMJD2D, also known as KDM4D (lysine (K)-specific demethylase 4D), using two KLH-conjugated synthetic peptides containing a sequence from the central region of the protein
Format: Aqueous solution
Formulation: Whole antiserum from rabbit containing 0.05% azide
Immunogen: synthetic peptide
Purification: Whole serum
Storage Stability: Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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