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Applications: Useful as a reference antibody for screening LAG-3 neutralizing antibodies.
Assay Conditions: Anti-LAG3 Neutralizing Antibody was added at various concentrations to the LAG-3/NFAT Reporter Jurkat cells (BPS Cat. #71278) co-cultured with superantigen-loaded Raji cellsand luciferase activity was measured using ONE-Step Luciferase Assay System (BPS Cat. #60690)
Description: Neutralizing recombinant human antibody recognizing the Lag-3 binding region.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Purification: Protein A affinity chromatography from HEK293 supernatants.
Storage Stability: Stable for at least 12 months at -80°C. Avoid freeze/thaw cycles. Store diluted antibody with carrier protein (BSA 0.1 - 0.5%) at 4°C, stable for 2 weeks.
Uniprot: P18627
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Byrnes, et al., Cytometry A. 2012 Sep;81(2):165-8. 2. Di Carlo, et al., J. Pathol. 2005 Jan; 205(1):82-91.