Products from BPS Bioscience require a minimum order value above 400€
Applications: WB, DB, ELISA, IP, ICC
Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Clone Number: clone Myc.A7/M19
Cross Reactivity: Works in human cells; predicted to work in all proteins containing the Myc tag sequence motif, regardless of species.
Description: Affinity purified Anti-Myc, clone Myc.A7/M19, Mouse IgG1. Recognizes both N-terminal or C-terminal Myc-tagged fusion proteins. Applications: Western Blot (1:1000 - 3000), Dot Blot, ELISA, Immunocytochemistry (1:500-2000), Immunoprecipitation (1:200), In Situ Hybridization. Optimal dilutions/concentrations should be determined by the end user.
Format: Aqueous buffer solution
Formulation: 1 mg/ml in 10 mM PBS, pH 7.2, with 0.05% NaN3
Immunogen: Synthetic peptide: EQKLISEEDL, corresponding to a.a. 410-419 of Human Myc tag conjugated to KLH.
Purification: Affinity chromatography
Storage Stability: Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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