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Applications: ELISA (1:500)
Assay Conditions: Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against mouse PHC2 (Cat. # 25307). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:33,300.
Background: PHC2 (UniProtKB/Swiss-Prot entry Q8IXK0) is a component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. The PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of lysine 119 on histone H2A, rendering chromatin heritably changed in its expressibility.
Concentration: 100 µl
Description: Polyclonal antibody raised in rabbit against mouse PHC2 (polyhomeotic homolog 2) using two KLH-conjugated synthetic peptides containing a sequence from the central region of the protein.
Format: Aqueous solution
Formulation: Whole antiserum from rabbit containing 0.05% azide
Immunogen: synthetic peptide
Purification: Whole serum
Storage Stability: Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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