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Applications: WB, DB, ELISA, IP, ICC, FS, PS
Background: The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 a.a. (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
Clone Number: clone E10/V4RR
Cross Reactivity: Works in human cells; predicted to work in all proteins containing the V5 tag, regardless of species.
Description: Affinity purified Monoclonal Anti-V5-Tag, clone E10/V4RR, mouse IgG1. Recognizes V5 (GKPIPNPLLGLDST) tagged fusion proteins. Affinity purified from culture supernatant of hybridoma cells. Applications: Western Blot (1:1000-3000), Dot Blot, ELISA (1:5000-10000), Immunocytochemistry (1:500-2000), Immunoprecipitation , Frozen sections (1:500-2000), Paraffin sections. Optimal dilutions/concentrations should be determined by the end user.
Format: Aqueous buffer solution
Formulation: 1 mg/ml in 10 mM PBS, pH 7.2, with 0.05% NaN3
Immunogen: Synthetic peptide: GKPIPNPLLGLDST
Purification: Protein A Affinity Chromatography
Storage Stability: Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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