Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: A 25 µl ACLY reaction is conducted in a buffer containing 40 mM Tris (pH 8.0), 10 mM MgCl
2, 2 mM DTT, 2 mM potassium citrate, 0.1 mM CoA and 6-100 ng human ACLY at 30°C for 60 min. Enzyme activity was assayed using ADP-Gloreagents (Promega)
Description: Human ATP citrate lyase (ACLY) (GenBank Accession No. NM_001096), full length, with N-terminal GST-tag, MW=147 kDa, expressed in baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 3 mM DTT, 10% glycerol
Genbank: NM_001096
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P53396
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Migita T. et al., Cancer Res. 68 (20), 8547- 8554 (2008).
2. Ramakrishna S. et al., Biochemistry 29 (33), 7617-7624 (1990). Application Reference:
ATP citrate lyase mediates resistance of colorectal cancer cells to SN38 (2013)
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