Clone: GRH1.
Conjugate: PerCP.
Immunogen: human beta2-microglobulin.
Storage Buffer: The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
Recommended Usage: β2 Microglobulin, clone GRH1, is an mAb intended for identification of B2M protein, component of the MHC I, involved in presentation of peptide antigens. It is effective for direct IF staining of human tissue for FCM analysis using β1 μg/106 cells.
Description: This antibody reacts with the beta2-microglobulin (B2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble B2-microglobulin. In the immunoprecipitation test the GRH1 two bands were precipitated on SDS-PAGE analysis of 43 kDa and 12 kDa corresponding to the heavy chain of the HLA-A, B and C antigens encoded by a gene on chromosome 6, and the beta 2-microglobulin which is a non-glycosylated protein noncovalently bound to the heavy chain that is encoded by a gene on chromosome 15 (Entrez Gene (human): 15q21-q22.2).
β2M plays an essential role both in governing MHC class I molecules stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8+ T cells.
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