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Applications: Useful for studying protein binding and screening small molecular inhibitors
Description: Human secreted B7-H2, also known as B7 homolog 2, CD275, ICOSL, ICOSLG, and B7-related protein (B7RP), GenBank Accession No. NM_001283050, a.a 19-258, with C-terminal Avi-His-Tag and expressed in a HEK293 cell expression system. This protein is enzymatically biotinylated using AviTag™ technology. MW = 31 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_001283050
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: Avi-His-Tag, Biotin-Labeled
Uniprot: O75144
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Guillonneau, C., et al., Transplantation. 2005; 80(4): 546-554.
2. Fan, X., et al., J. Exp. Med. 2014; 211(4): 715-725.
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