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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done in Kinase buffer containing 0.2 mM DTT using Poly-(Tyr,Glu) substrate (0.2 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was measured using Kinase-Glo reagent (Promega)
Background: The BMX gene encodes a non-receptor tyrosine kinase, which may play a role in the growth and differentiation of hematopoietic cells (1). The BMX gene is located on chromosomal band Xp22.2 between the DXS197 and DXS207 loci. Interestingly, chromosome X also contains the closest relative of BMX, the BTK gene, implicated in X-linked agammaglobulinemia. BMX, is found to induce activation of the Stat signaling pathway (2).
Description: Recombinant full-lengthhuman cytoplasmic tyrosine-proteinkinase BMX (BMX), also known as bonemarrow tyrosine kinase gene inchromosome X protein, epithelial andendothelial tyrosine kinase, ETK, andNTK38 was expressed by baculovirus inSf9 insect cells using an N-terminal GSTtag, a.a. 1-675 (end). The geneaccession number is NM_001721(variant 2).
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25%glycerol
Genbank: NM_001721
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P51813
Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Tamagnone, L. et al. Oncogene. 1994 Dec;9(12):3683-8.
2. Saharinen, P. et al. Blood. 1997 Dec 1;90(11):4341-53