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Applications: Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Assay Conditions: BRD2 is incubated with biotinylated acetyl-histone peptide substrate (BPS #33000) in BRD assay buffer (BPS #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA® Nickel Chelate acceptor beads (PerkinElmer) are added, followed by Alphascreen® Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour minute incubation at RT, Alphacounts are measured.
Description: Human Bromodomain containing 2, also known as BRD2 or RNF3, GenBank Accession No. NM_005104, a.a. 339 - 459 corresponding to bromdomain 2 with N-terminal His-tag, MW = 15.1 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 0.05% Tween-20, and 10% glycerol
Genbank: NM_005104
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P25440
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Umehara, T., et al., FEBS Lett. 2010 Sep 24; 584(18): 3901-3908.
2. Shang, E., et al., J Cell Biochem. 2011 Oct; 112(10): 2784-2793.
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