Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 49-460
Applications: Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed according to BRD4 (BD1+BD2) TR-FRET Assay Kit (BPS Bioscience #32612) with BRD4 (BD1+BD2) Recombinant titrated at various concentrations as a competitive antagonist to BRD4 (BD1+BD2) GST-Tag Recombinant (BPS Bioscience #31044).
Description: Human Bromodomain containing 4, also known as BRD4, HUNK1, or MCAP, GenBank Accession No. NM_058243, a.a. 49-460 corresponding to bromodomains 1 and 2, with no tag, MW = 46.9 kDa, expressed in an E. coli cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 7.5, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol.
Genbank: NM_058243
Storage Stability: Store at -80°C. Stable for >6 months when stored as directed.
Tags: none
Uniprot: O60885
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Rodriguez, RM, et al., J Mol Med (Berl). 2012 May; 90(5): 587-95.
2. Voigt, P, et al., Genome Biol. 2011 Nov 29;12(11):133.