Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 135-242
Applications: Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed using BRD9 Inhibitor Screening Assay Kit, Cat. # 32519. BRD9 protein is incubated with biotinylated acetyl-histone peptide substrate BET Bromodomain Ligand in BRD9 assay buffer in a 10 µl reaction for 30 min at RT. AlphaLISA® GSH acceptor beads (PerkinElmer) were then added, and the mixture was incubated for 30 minutes at room temperature (RT). Alphascreen® Streptavidin-conjugated donor beads (PerkinElmer) were then added. After 10 minute incubation at RT, Alphacounts are measured.
Description: Human Bromodomain containing 9, also known as BRD9, GenBank Accession No. NM_023924, a.a. 135 - 242 corresponding to single bromodomain with N-terminal GST-tag, MW = 39 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_023924
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9H8M2
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Scotto, L., et al., Mol. Cancer. 2008 Jun 17; 7:58
2. Kang, J.U., et al., Cancer Genet Cytogenet. 2008 Apr 1; 182(1): 1-11.
English
Deutsch
