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Encompassing Amino Acids: 257-382
Applications: Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed according to BRD4 (BD1+BD2) TR-FRET Assay Kit (BPS Bioscience #32612) with BRD4 (BD1+BD2) Recombinant titrated at various concentrations as a competitive antagonist to BRD4 (BD1+BD2) GST-Tag Recombinant (BPS Bioscience #31044).
Description: Human Bromodomain testis-specific protein, also known as BRDT, BRD6, and CT9, GenBank Accession No. NM_207189, a.a. 257 - 382 corresponding to bromodomain 2 with N-terminal GST-tag, MW = 41.7 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Genbank: NM_207189
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q58F21
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Pivot-Pajot C., et al., Mol Cell Biol. 2003 Aug; 23(15): 5354-65.
2. Moriniére J., et al., Nature. 2009 Oct 1; 461(7264):664-8.
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