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Encompassing Amino Acids: 23-787
Application: Useful for binding assays.
Assay Conditions: This protein was tested using an Alpha-LISA™ binding assay. A 10 µl reaction mix containing E-cadherin in 1x PP-02 Buffer was incubated with Cadherin17, Fc-fusion (IgG1), Avi-Tag, Recombinant for one hour at room temperature. Protein A acceptor beads were added, and the reaction was incubated for 30 minutes, followed by the addition of Nickel Donor beads. A-counts were measured. The net A-count signal is proportional to E-cadherin-Cadherin17 binding.
Background: CHD17 (cadherin 17), also known as LI (liver-intestine)-cadherin is a unique member of the cadherin superfamily of proteins, as it has seven instead of the five typical cadherin domains. It is a calcium-dependent membrane-associated glycoprotein normally expressed on epithelial cells of the small intestine and colon, where it regulates intercellular adhesion. Upregulation of this protein is found in gastric cancer, colorectal and pancreatic cancer, amongst others. CHD17 has become a therapeutic target of interest, with studies using monoclonal antibodies, ADC (antibody drug conjugates) and CAR-T cells resulting in promising outcomes. More recently nanobodies, with their smaller size and higher potential to penetrate tumors, have also been developed. A bispecific T cell engager, ARB202, has also shown great promise in vitro. CHD17 is thus a target with great potential and future studies will continue to open new avenues of treatment around it.
Concentration: 1.83 mg/ml
Description: Recombinant human cadherin17 (CDH17), encompassing amino acids 23-787. This construct contains at the C-terminus the Fc domain of a human IgG1, followed by an Avi-Tag™. This protein was affinity purified.
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Genbank: NM_004063.4
Host Cell Line: HEK293
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: C-terminus IgG1
Uniprot: Q12864
Warnings: Avoid multiple freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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