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Encompassing Amino Acids: Full-length
Application: Useful for studying calmodulin-dependent kinase kinetics in vitro.
Assay Conditions: Varying amounts of calmodulin 25 ng CAMK2, 200 ng/µl autocamtide-2 peptide, 0.4 mM CaCl2, 50 µM ATP and trace [33P]-ATP in 25 µl of reaction volume for 15 min at 30°C.
Description: Human Calmodulin 1, also known as CALM1, CALML2, CAMI, DD132, PHKD, GenBank Accession No. NM_006888, full-length with an N-terminal His-tag, expressed in an E. coli cell expression system. MW = 17 kDa.
Format: Aqueous buffer solution.
Formulation: 50 mM sodium phosphate,pH 7.0, 300 mM NaCl, 150 mM imidazole,0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
Genbank: NM_006888
Purity: ≥90%
Storage Stability: >6 months at –80°C.
Tags: N-terminal His-tag
Uniprot: P62158
Warnings: Avoidfreeze/thaw cycles. Storing diluted protein isnot recommended, if necessary, use carrierprotein (BSA 0.1 – 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Li, H. et al., FEBS Lett. 2004; 13(559): 175-180.
2. Huang, M. et al., Cell Stress Chaperones. 2009; 14(4): 343-353.
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