Application Note: Calmodulin 1 Protein is stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. Calmodulin 1 Protein is suitable for use in Western Blot. Expect a band approximately ~ 17kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Concentration Value: 0.2 µg/µL
Western Blot Dilution: User Optimized
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Recombinant full-length human Calmodulin1 was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.
Background: Calmodulin 1 is a member of calcium-modulated proteins which is present in the cytosol and on membranes facing the cytosol and has a high affinity for calcium. Calmodulin 1 has 4 calcium-binding domains and plays a role in cell growth, cell cycle, signal transduction and the synthesis and release of neurotransmitters. Calmodulin can bind to the epidermal growth factor receptor at its cytosolic juxtamembrane region and this inhibits its tyrosine kinase activity (1). A number of other proteins including HSP70 have been shown to interact with Calmodulin 1 in a cell-phase-specific manner (2). Calmodulin 1 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cardiovascular Disease, ERK/MAPK Pathway, Neurobiology, and PKA/PKC Pathway research.
Low Endotoxin: No
Other: Kinase Assay-User Optimized
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