Target: Calnexin.
Conjugate: ATTO 390.
Product Type: Polyclonal.
Immunogen: Dog Calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
Swiss-Prot: P24643.
Purification: Protein A Purified.
Storage Buffer: PBS pH7.2, 50% glycerol, 0.09% sodium azide.
Concentration: 1 mg/ml.
Specificity: Detects the C-terminal domain of Calnexin ~90kDa. Weak detection in Chicken, Drosophila, and Xenopus tissues.
Cellular Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Membrane, Melanosome.
Scientific Background: Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
References: 1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science. 263(5145): 387-90.2. Tjoelker L.W., et al. (1994) Biochemistry. 33: 3229.3. Schrag J. et al. (2001) Molecular Cell. 8(3): 633-644.4. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813-40819.5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460.6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103.7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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