Application Note: Anti-Calnexin-CT Antibody has been tested in ICC, IHC and in WB. Expect a band approximately ~90kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Concentration Value: 85 mg/mL
Western Blot Dilution: 1:2000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Calnexin-CT Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of dog calnexin. Identical to human, mouse and rat calnexin sequences over these residues.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Anti-Calnexin -CT Antibody was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest cross-reactivity with Calnexin -CT from Human, Monkey, Mouse, Rat, Bovine, Chicken (weak), Dog, Guinea pig, Hamster, Pig, Quail, Rabbit, Sheep, Drosophila (weak), and Xenopus (weak) based on 100% homology with the immunizing sequence. Cross-reactivity with Calnexin -CT from other sources has not been determined. Cell Signaling and Organelle Marker research.
Background: Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90. It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail. Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention, calnexin has positively charged cytosolic residues that do the same thing. Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglycosylated glycans. Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin.
Low Endotoxin: No
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