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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: CAMKIβ is thought to be involved in a variety of developmental processes including development of the central nervous system (1). CAMKIβ2, an isoform of mCAMKIβ, is mainly present in the nervous system, including brain, spinal cord, trigeminal ganglion, and retina. Within the CNS, the expression of CAMKIβ2 is detected in the mantle zone suggesting its possible involvement in the differentiation of neurons (2).
Description: Recombinant mouse CAMK1β, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_012040
Host Cell Line: Sf9 cells
Purity: ≥75%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Gardner,H.P. et al: Cloning, characterization, and chromosomal localization of Pnck, a Ca(2+)/calmodulin-dependent protein kinase. Genomics 63 (2), 279-288 (2000).
2. Ueda,T. et al: Distribution and intracellular localization of a mouse homologue of Ca2+/calmodulin-dependent protein kinase Ibeta2 in the nervous system. J. Neurochem. 73 (5), 2119-2129 (1999).
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