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Encompassing Amino Acids: 59-414
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 15 mM Trizma hemisulfate, pH 7.6, and 3 mM p-Nitrophenyl acetate (PNPA). Incubate for 30 minutes at room temperature. Absorbance is measured at 410 nM.
Background: CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. Expression of the membrane-bound carbonic anhydrase, CA IX, has been reported in hypoxic tumors where its activity is linked to promoting survival of tumor cells and aiding cancer progression. This is thought to occur through the carbonic anhyrase-mediated production of bicarbonate ions, which neutralizes the abnormally acidic pH of the extracellular environment induced during hypoxia. Furthermore, as CA IX is a hypoxia- and HIF-1-inducible enzyme, its expression can be used as a biomarker for hypoxic tumors.
Description: Human carbonic anhydrase IX (CA IX), also known as CA9, (GenBank Accession No. NM_001216), a.a. 59-414 with C-terminal His-tag, MW = 39.7 kDa, expressed in a baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 266 mM imidazole and 20% glycerol
Genbank: NM_001216
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: Q16790
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Hilvo, M., et al., J Biol Chem. 2008 Oct 10;283(41):27799-809.
2. Chadwick, A.L., et al., Cell Cycle. 2013 Jul 30;12(16):2534.
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