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Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Description: Human Casitas B-lineage lymphoma proto-oncogene b (CBL-B), also known as E3 ubiquitin-protein ligase CBL-B, RING finger protein 56, and SH3- binding protein CBL-B with Y363F mutation GenBank Accession No. NM_170662, a.a. 39 - 426 with N-terminal His-tag and N-terminal Avi-tag, expressed in an E. coli expression system. This protein was enzymatically biotinylated in vivo using AviTag™ technology. MW=48 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Hepesl, pH 7.4, 120mM NaCl, 20% glycerol, 200 mM imidazole
Genbank: NM_170662
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag, N-terminal Avi-tag
Uniprot: Q13191
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Jeon, M.S., et al., Immunity. 2004; 21(2):167-177.2. Chiang, Y.J. et al., Nature. 2000; 403:216-220.
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