Clone: 104D2.
Conjugate: PE-Cyanine7.
Immunogen: MOLM-1 megakaryocytic cell line.
Storage Buffer: The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
Recommended Usage: Immunostep’s CD117, clone 104D2 is a mAb intended for the identification of lymphoid progenitor. This reagent is effective for direct IF staining of human tissue for FCM analysis using ≤1 μg/106 cells.
Description: This antibody reacts with the CD117 antigen, which is expressed within the haematopoeitic compartment on approximately 50 % of CD34+ progenitors engaged in erythrocytic, myelo-monocytic and megakaryocytic differentiation. The 104D2 monoclonal antibody reacts with human CD117, also known as c-Kit, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation.
Although CD117 is primarily a marker for non-lymphoid progenitor, it has been reported to be detected on early lymphoid progenitor. CD117 expression has been found on a small subset of resting NK cells (CD56 bright), and about 30% of immature CD3–/CD4–/CD8– thymocytes. CD117 is also expressed on mast cells and detected on non-hematopoietic cells such as reproductive system, melanocytes and embryonic brain.
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