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Applications: Useful for studying protein binding and screening small molecular inhibitors
Description: Human secreted CD137, Fc fusion protein, also known as Tumor Necrosis Factor Receptor Superfamily member 9, TNFRSF9, and ILA, GenBank Accession No. NM_001561, a.a. 24-186, expressed in a HEK293 cell expression system, fused at the C-terminus to the Fc portion of human IgG1 and enzymatically biotin-labeled using Avi-tag™ technology. MW = 45.9 kDa (monomer). Biotinylation is confirmed to be >90%. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Endotoxin Level: <1 EU/µg
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Genbank: NM_001561
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: C-terminal Fc fusion, Avi-Tag
Uniprot: Q07011
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Wilcox, R.A., et al., J. Clin. Invest. 2002; 109(5): 651-659.2. Foell, J. et al., J. Clin. Invest. 2003; 111(10): 1505-1518.
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