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Encompassing Amino Acids: 24-186
Applications: Useful for studying protein binding and screening small molecular inhibitors
Description: Human secreted CD137, Fc fusion protein, also known as Tumor Necrosis Factor Receptor Superfamily member 9, TNFRSF9, and ILA, GenBank Accession No. NM_001561, a.a. 24-186 expressed in a HEK293 cell expression system. MW = 44 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Endotoxin Level: <1 EU/µg
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Genbank: NM_001561
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal Fc fusion (Human IgG1)
Uniprot: Q07011
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Wilcox, R.A., et al., J. Clin. Invest. 2002; 109(5): 651-659.2. Foell, J. et al., J. Clin. Invest. 2003; 111(10): 1505-1518.