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Applications: Screen and profile inhibitors of CD137 binding to CD137L.
Background: CD137, also known as 4-1BB, is a prototype co-stimulatory molecule expressed on T cells. CD137 interaction with its cognate ligand, CD137L, also known as 4-1BBL, results in increased T cell activation and proliferation. Accumulating evidence shows a role for CD137:CD137L signaling in inflammation, suggesting that inhibition of this pathway may provide a therapeutic avenue to treat autoimmune and inflammatory diseases.
Description: The CD137:CD137L [Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD137:CD137L interaction. The assay takes advantage of the highly sensitive detection of biotin-labeled CD137L by streptavidin-HRP. First, CD137 is coated on a 96-well plate. Next, CD137L-biotin is incubated with CD137. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
Storage Stability: This assay kit will perform optimally for up to one year from date of receipt when the materials are stored as directed.
Supplied As: The CD137:CD137L[Biotinylated] Inhibitor Screening Assay Kit comes in a convenient 96-well format, with biotin-labeled CD137L, purified CD137, streptavidin-labeled HRP, and assay buffer for 100 reactions.
Uniprot: CD137: Q07011; CD137L: P41273
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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