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Applications: Isolate CD19-expressing B cells from a mixed population such as PBMCs. Positively selected cells may be used for downstream applications such as genomic analysis, expression assays, protein isolation and flow cytometry.
Background: B cells are lymphocytes that play a critical role in the humoral immune response. These cells produce antibodies, present antigens, and secrete cytokines. CD19 is a canonical marker of B cells and is present at most stages of B cell development. In PBMCs derived from healthy individuals, about 5-10% of the cells are CD19+ B cells.
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: This kit provides enough reagents and materials for isolation from up to 1 x 108 or 1 x 109 PBMCs. It is possible to use this kit for multiple isolations from smaller PBMC samples.
Uniprot: P15391
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-1
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