Products from BPS Bioscience require a minimum order value above 400€Application: Study and screen compounds that inhibit the binding of CD200R1 to CD200 for drug discovery in high throughput screening (HTS) applications.
Background: CD200 (Cluster of Differentiation 200) and its receptor, CD200R, are cell surface proteins that play a crucial role in immune regulation and tolerance. The CD200-CD200R pathway is primarily involved in inhibiting immune responses, helping to maintain immune homeostasis and prevent excessive inflammation. In many cancers, tumor cells overexpress CD200, which interacts with CD200R on immune cells, particularly myeloid cells like macrophages and dendritic cells. This interaction leads to the suppression of immune responses against the tumor, effectively promoting immune evasion. Neutralizing antibodies against CD200 or CD200R can block this interaction, preventing the inhibitory signal from being delivered to immune cells. As a result, the anti-tumor immune response is enhanced, leading to improved recognition and elimination of tumor cells by the immune system. The development of strategies targeting this complex holds great potential in cancer therapy.
Contraindications: The CD200R1:CD200 Binding Assay Kit is compatible with up to 1% final DMSO concentration.
Description: The CD200R1:CD200 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA designed to assess the efficacy of inhibitors of the binding between CD200R1 (CD200 receptor 1) and CD200 (cluster of differentiation 200) protein for screening and profiling applications. This kit comes with enough purified CD200R1 (amino acids 29-265) and biotinylated CD200 (amino acids 31-232), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. A 96-well plate is coated with CD200R1 protein. After blocking, biotinylated CD200 is added in an optimized assay buffer. Next, unbound biotinylated CD200 is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to CD200 binding to CD200R1.Need us to run inhibitor screens or profile your compounds against IL-17RA:IL-17A[Biotin]? Check out our
Immunotherapy Biochemical Screening Services.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Uniprot: Q8TD46, P41217
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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