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Encompassing Amino Acids: 21-192
Applications: Useful for studying protein binding and screening small molecular inhibitors
Description: Human secreted CD27, Fc fusion protein, also known as Tumor Necrosis Factor Receptor Superfamily Member 7, TNFRSF7, and T14, GenBank Accession No. NM_001242, a.a. 21-192 expressed in a HEK293 cell expression system. MW = 45.8 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Endotoxin Level: <1 EU/µg
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Genbank: NM_001242
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal Fc fusion (Human IgG1)
Uniprot: P26842
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Hendricks, J. et al., Nat. Immunol. 2000; 1: 433-440.2. Ruprecht, C.R. et al., J. Exp. Med. 2005; 201(11): 1793-1803.