Clone: UCHL1.
Conjugate: FITC.
Immunogen: Human IL-2-dependent T-cell line.
Storage Buffer: The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
Recommended Usage: CD45RO, clone UCHL1 is a mAb intended for identification/enumeration of CD45RO present on approx. 40% of peripheral blood T lymph using FCM. This reagent is effective for direct IF staining of human tissue for FCM analysis using ≤1 μg/106 cells.
The CD45RO antigen is present at low density early in the T-lymphocyte maturation cycle. Upon activation by phytohemagglutinin (PHA) or alloantigen, naive T lymphocytes first acquire CD45RO and then lose CD45RA. When these activated T lymphocytes are rechallenged, the cells that exhibit a secondary response are primarily CD45RO+ , leading to the concept that CD45RO + cells are a primed population of memory T lymphocytes.
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