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Encompassing Amino Acids: 19-139
Applications: Useful as a positive control for Western blot analysis.
Description: Human CD47, Fc fusion protein, also known as leukocyte surface antigen CD47, integrin-associated protein (IAP), OA3, and MER6, GenBank Accession No. NM_001777, a.a. 19-139, with C-terminal Avi-Tag, expressed in a HEK293 cell expression system. The purified protein (40 kDa) was covalently labeled by streptavidin (~13 kDa). Protein runs at a higher MW by SDS-PAGE due to glycosylation and streptavidin labeling.
Format: Aqueous buffer solution
Formulation: 40 mM Tris, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_001777
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal Fc fusion (Human IgG1), Avi-Tag
Uniprot: Q08722
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Vernon-Wilson, E.F. et al., Eur. J. Immunol. 2000; 30(8): 2130-2137.
2. Majeti, R. et al., Cell. 2009; 138(2): 286- 299.