Clone: 143-44.
Conjugate: FITC.
Immunogen: T-lymphocyte differentiation antigen.
Storage Buffer: The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
Recommended Usage: CD8, clone 143-44, is a mAb intended for the identification/enumeration of human T cells suppressor/cytotoxic using FCM. This reagent is effective for direct IF staining of human tissue for FCM analysis using ≤1 μg/106 cells.
Description: This antibody reacts with the CD8-antigen (T8-antigen),which is expressed on human T lymphocytes. The monoclonal antibody reacts with 20-30% of human peripheral T lymphocytes. The monoclonal antibody reacts with T lymphocytes with suppressor-cell activity in pokeweed mitogen- stimulated immunoglobulin production, as was shown in separation experiments (i.e. "panning"). The monoclonal antiody does not react with B-cells, monocytes, granulocytes and platelets.
30/32 kD MW lymphocyte surface antigen identified by monoclonal antibodies belonging to the CD8 cluster on a sub-population of peripheral blood T lymphocytes, 60% of thymocytes, and a limited number of malignancies of T cell origin. Normal B lymphocytes, monocytes or granulocytes do not express surface CD8 antigen
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