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Encompassing Amino Acids: CDK16: 107-496(end)Cyclin Y: 2-341(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 50 ng/ml BSA, 2 mM ATP, 200 µg of MBP (or Histone H1) as substrate. Add [33P]-ATP and incubate at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Description: CDK16/Cyclin Y complex of human CDK16, also known as PCTK1 and PCTAIRE1 GenBank Accession No. NM_006201, a.a. 107-496(end) with N-terminal GST-tag, MW=66 kDa, and human Cyclin Y, also known as CCNY, CBCP1, CCNX, and CFP1, GenBank Accession No. NM_145012, a.a. 2-341(end) with N-terminal GST-tag. MW= 64 kDa. Co-expressed in a Baculovirus infected Sf9 cell expression system.
Formulation: 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: CDK16: NM_006201; Cyclin Y: NM_145012
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: CDK16: N-terminal GST-tagCyclin Y: N-terminal GST-tag
Uniprot: CDK16: Q00536Cyclin Y: Q8ND76
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Meyerson, M. et.al, EMBO J. 11: 2909-2917, 1992.
2. Knight, J. C. et.al, Cytogenet. Cell Genet. 71: 155-157, 1995.
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