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Applications: Useful for the study of bromodomain binding assays, screening bromodomain inhibitors and selectivity profiling.
Contraindications: Only limited amounts of DMSO can beincluded, as it has been shown to disrupt BRD-ligand interaction. Avoid green and bluedyes that absorb light in the AlphaScreen® signal emission range (520-620 nm), such asTrypan Blue.
Description: The CECR2 Inhibitor Screening Assay Kit is designed to measure the inhibition of CECR2 binding to its substrate. The key to the CECR2 Inhibitor Screening Assay Kit is the highly specific binding of the CECR2 bromodomain to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing CECR2 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
Storage Stability: Stable at least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: Kit comes in a convenient AlphaLISA® format, with biotinylated histone peptide substrate, assay buffer, detection buffer and purified CECR2 (Cat Eye Syndrome Chromosome Region 2) bromodomain to perform a total of 384 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Lee S-K, et al., Mol. Cells 2012, 34(1):85-91.
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